Received: 16 July 2019; Published: 30 October 2019

Status of humoral immune factors in peripheral blood of fronto-orbital trauma patients late after surgery with the use of biocomposite  

О.D. Bondarchuk1,2, V.V. Kishchuk1, О.F. Melnykov3, М.D. Tymchenko3, N.D. Didyk2

1 Pirogov National Medical University of Vinnytsia; Vinnytsia (Ukraine)

2 Pirogov Regional Clinical Hospital of Vinnytsia; Vinnytsia (Ukraine) 

3 Prof. Kolomiichenko Institute of Otolaryngology, NAMS of Ukraine; Kyiv (Ukraine)

E-mail: eskulap20009@gmail.com

TO CITE THIS ARTICLE: Bondarchuk О.D., Kishchuk V.V., Melnykov О.F., Tymchenko М.D., Didyk N.D. Status of humoral immune factors in peripheral blood of fronto-orbital trauma patients late after surgery with the use of biocomposite. J.ophthalmol.(Ukraine).2019;5:37-41.  http://doi.org/10.31288/oftalmolzh201953741


Purpose: To identify the influence of biocomposite on immune system status in front bone trauma (FBT) patients at 9 months or later after surgery involving the use of the composite material.

Material and Methods: Twenty FBT patients underwent an examination 9 months or later after surgery. They were divided into two groups, group A with a problematic clinical course and group B with an uneventful clinical course during follow-up. The control group was composed of 10 age-matched and practically healthy individuals. Serum levels of immunoglobulin (Ig)M, IgG, IgA and IgE, interleukins (IL)-1 and IL-10, transforming growth factor (TGF)-β, circulating immune complexes (CIC), and gamma interferon (IFN-γ), and agglutination antibodies to antigens of allogeneic connective tissue were measured. Non-parametric Wilcoxon U test and Student t-test were used for statistical analysis.

Results: Activation of pro- and anti-inflammatory interleukins and no changes in the levels of immunoglobulins and CIC were found in group A, but not group B or the controls. In addition, patients from group A exhibited decreased serum levels of TGF-β and IFN-γ, which may be considered as pathogenetically significant factors involved in regeneration processes. No humoral autoimmune response to connective tissue antigens was found.

Conclusion: Two disease course variants were found in the late phase after FBT. Increased serum levels of pro- and anti-inflammatory interleukins and decreased serum levels of TGF-β and, especially, IFN-γ, were observed in patients with problematic regeneration. No humoral autoimmune response to connective tissue antigens was found in either group of patients with FBT. Stress mechanisms might be involved in decreased immunity levels in these patients.

Keywords: trauma, immunoglobulins, cytokines, growth factor, immune complexes, humoral autoimmune responses


1.Gorbunov VI, Likhterman LV, Gannushkina IV. [Immunology of traumatic brain injury]. Ulianovsk:SVNTS; 1996. Russian.

2.Lysianyi NI, Pedachenko EG, Kadzhaia NV. [Features of autoimmune responses developing in recurrent TBI]. Immunologiia ta allergologiia. 2006;3:53–6. Ukrainian.

3.Matricon J, Barnish N, Ardid D. [Immunopathogenesis of inflammatory bowel disease]. Med Sci (Paris). 2010 Apr;26(4):405-10. doi: 10.1051/medsci/2010264405. French.

Crossref    PubMed

4.Zhuang J, Shackford SR, Schmoker JD, Anderson ML. The association of leukocytes with secondary brain injury. J Trauma. 1993 Sep;35(3):415-22.

Crossref    PubMed  

5.Kishchuk VV, Bondarchuk OD, Dmytrenko IV, Lobko KA. [Changes in humoral systemic immunity as an objective criterion of the rehabilitation process in patients with frontal basilar trauma]. Zhurnal vushykh, nosovyjh i gorlovykh khvorob. 2018;5:45. Ukrainian.

6.Kishchuk VV, Bondarchuk OD. [Using Syntekist biocomposites to repair bone defects in ear, nose and throat organs]. Zhurnal vushykh, nosovyjh i gorlovykh khvorob. 2007;5c:43–4. Ukrainian.

7.Gubler ЕV. [Informatics in pathology, clinical medicine and pediatrics]. Leningrad:Meditsina;  1990. Russian.

8.Demianov AV, Kotov AIu, Simbirtsev AG. [Diagnostic value of studying cytokine levels in clinical practice]. Cytokines and inflammation. 2003;3:20–8. Russian.

9.Drannik GN. [Clinical immunology and allergology]. Kyiv:Polygraph plus; 2010. Russian.

10.Kabat EA, Mayer MM. [Experimental immunochemistry]. Moscow:Mir; 1968. Russian.

11.Kovalchuk LV, Gankovskaya LV, Meshkova RY. [Clinical immunology and allergology with the basics of general immunology]. Moscow: GEOTAR–Media; 2011. Russian.

12.Nasonov EL. [Methodological aspects of measurements of circulating immune complexes with the use of polyethylene glycol]. Terapevticheskie arkhivy. 1987;4:38–45. Russian.

13.Melnykov OF, Zabolotnyi DI, Maliarenko TV, Prylutska OD. [Rynital in the rational pharmacotherapy of seasonal allergic rhinitis]. Kyiv:Vistka Ltd; 2015. Ukrainian.

14.Iakovenko VD. [Experimental and clinical grounding of the allergic diagnosis of chronical tonsillitis]. [Abstract of Cand Sc (Med) Thesis]. Kyiv: Prof. Kolomiichenko Institute of Otolaryngology; 1985. Russian.


The authors certify that they have no conflicts of interest in the subject matter or materials discussed in this manuscript.