J.ophthalmol.(Ukraine).2019;4:43-48.

http://doi.org/10.31288/oftalmolzh201944348

Comparing structural changes in the rabbit’s uveal tract after the use of a radiowave surgery unit versus a cutting tool

O.V. Khomiakova, Ophthalmologist; V.V. Vit, Dr Sc (Med), Prof.; A.P. Maletskyi, Dr Sc (Med)

SI " The Filatov Institute of Eye Diseases and Tissue Therapy of the NAMS of Ukraine”; Odesa (Ukraine)

E-mail:  maletskiy@filatov.com.ua

TO CITE THIS ARTICLE: Khomiakova OV, Vit VV, Maletskyi AP. Comparing structural changes in the rabbit’s uveal tract after the use of a radiowave surgery unit versus a cutting tool. J.ophthalmol.(Ukraine).2019;4:43-48. http://doi.org/10.31288/oftalmolzh201944348

 

Background: Organ-saving treatment strategies for uveal melanoma are still the most common therapeutic approach. Since there is insufficient experience of the use of radiowave surgery technologies, further research is required regarding (a) the effect of a radiowave surgery unit on histology of the tumor and healthy ocular tissues and (b) the changes in post-operative wound healing after implementation of various intervention techniques.

Purpose: To assess the response of the sclera and uveal tract to the use of a radiowave surgery unit versus a cutting tool in animals.

Materials and Methods: The studies were performed on rabbits taken from the vivarium of the Filatov Institute. A total of 12 Chinchilla adult male rabbits (age, 4–6 months; 12 eyes) were used in the study. Six rabbits of the experimental group (Group 1) underwent monolateral excision of ocular structures (the iris, ciliary body and choroid) with a 4.0-MHz radiowave surgery unit (Surgitron, Ellman International) set on monopolar mode.

Results: Histomorphological studies of the ocular tissues (sclera, iris, ciliary body and choroid) in experimental rabbits demonstrated that after surgery with the use of a radiowave surgery unit, necrotic and degenerative changes were observed in the ocular tissues adjacent to the wound canal which had been preserved intraoperative, and these changes expanded to the retina. The changes in the tissues after the use of conventional cutting tools were less apparent.

Conclusion: After surgery with the use of a radiowave surgery unit, necrotic and degenerative changes developed in the ocular tissues (ciliary body, scleral and sunconjunctival tissue fragments) adjacent to the wound canal which had been preserved intraoperative. The above changes were maintained, tending to become more severe over 30 days.

Keywords: uvea, radiowave surgery, experimental model, histomorphological changes

References

1.Il’in EA. [Bioethics in human and animal studies]. Aviakosmicheskaia I ekologicheskaia meditsina. 2016;50(5):69-77. Russian.

2.Chua V, Aplin AE. Novel therapeutic strategies and targets in advanced uveal melanoma. Curr Opin Oncol. 2018 Mar;30(2):134-141. doi: 10.1097/CCO.0000000000000425.

3.Kozina EV, Kozina IuV, Gololobov VT, Kokh IA. [Uveal melanoma: major epidemiological aspects and risk factors]. Sibirskoie meditsinskoie obozreniie. 2014; 4(88): 57-64. Russian.

4.Yang J, Manson DK, Marr BP, Carvajal RD. Treatment of uveal melanoma: where are we now? Ther Adv Med Oncol. 2018 Feb 21;10:1758834018757175. doi: 10.1177/1758834018757175.

5.Smoliakova GP, Pikhovskaia IG, Luz’ianina IG, Sorokin EL. [Method for radiosurgical treatment of uveal melanoblastoma]. Russian. Available at http://www.findpatent.ru/patent/226/2265423.html

6.Korobov EN, Iarovoi AA, Gorshkov IM. [Primary endoresection of choroidal melanoma]. Sovremennye tekhnologii v oftalmologii. 2017;1(14):142-5. Russian.

7.Borovikov VP. [Statistica: the art of computerized data analysis]. St Petersburg: Piter;2003. Russian.

8.Gigliotti CR, Modorati G, Di Nicola M, Fiorino C, et al. Predictors of radio-induced visual impairment after radiosurgery for uveal melanoma. Br J Ophthalmol. 2018 Jun;102(6):833-839. doi: 10.1136/bjophthalmol-2017-310801.

9.Jang BS, Chang JH, Oh S, Lim YJ, Kim IH. Surgery vs radiotherapy in patients with uveal melanoma: Analysis of the SEER database using propensity score matching and weighting. Strahlenther Onkol. 2017 Nov;193(11):931-942. doi: 10.1007/s00066-017-1203-0.

10.Vazhenin AV, Panov IE, Semionova LE, et al. [Our first experience of CyberKnife treatment for choroidal melanoma]. Sibirskii onkologicheskii zhurnal. 2012; (1): 48-50. Russian.

11.Kim JH, Shin SJ, Heo SJ, et al. Prognoses and Clinical Outcomes of Primary and Recurrent Uveal Melanoma. Cancer Res Treat. 2018 Oct; 50(4): 1238–51.

12.Maletskiy AP, Homyakova OV. [Radiowave surgery and seleсtive еndarterial chemotherapy in treatment of patients with uveal melanoma]. In: Proceedings of the Joint Congress SOE/AAO. Geneva, 4-7 June 2011. p.139.

13.Pham C M, Custer PL, Couch SM. Comparison of primary and secondary enucleation for uveal melanoma. Orbit. 2017 Dec;36(6):422-427. doi: 10.1080/01676830.2017.1337183.

The authors certify that they have no conflicts of interest in the subject matter or materials discussed in this manuscript.