The effect of high intraocular pressure on enzymatic antioxidant system of uveal tissues in rabbits with experimental allergic uveitis
I.M. Mikheitseva, Dr. Sc. (Biol.); N.V. Bondarenko, a Postgraduate Student;
S.G. Kolmiichuk, a Research Fellow; T.I. Siroshtanenko, a Junior Research Fellow; M.M. Trofimov, Cand. Sc. (Vet.); L.V. Zagrodska, a Laboratorian
SI " The Filatov Institute of Eye Diseases and Tissue Therapy of the NAMS of Ukraine”; Odesa (Ukraine)
TO CITE THIS ARTICLE: Mikheitseva IM, Bondarenko NV, Kolmiichuk SG, Siroshtanenko TI, Trofimov MM, Zagrodska LV. The effect of high intraocular pressure on enzymatic antioxidant system of uveal tissues in the rabbits with experimental allergic uveitis. J.ophthalmol.(Ukraine).2019;4:57-63. http://doi.org/10.31288/oftalmolzh201945763
Background. Determining the influential mechanisms of high intraocular pressure on the development and course of non-infectious anterior uveitis is a relevant but understudied issue which requires a detailed study. Of particular interest, in this regard, are free radical mechanisms which can be a trigger for oxidative stress and cause damage to cell membranes in ocular tissues.
Purpose. To study the activity of antioxidant enzymes (glutathione peroxidase, superoxide dismutase, and catalase) in uveal tissues and chamber aqueous humor in rabbits with experimental non-infectious anterior uveitis with and without ocular hypertension.
Material and Methods. The experiment involved forty-one animals divided into 4 study groups: Group 1 (n = 10), ocular hypertension (OH); Group 2 (n = 10), uveitis (U); Group 3 (n = 12), (OH+U); Group 4 (n = 9), intact animals as control. Allergic uveitis was simulated using our method through the administration of bull serum albumin with a dose of 5 mg in previously sensibilized animals. To simulate ocular hypertension in the experimental groups 1 and 3, the animals were made a single 0.1 ml injection of 0.3% carbomer in the anterior chamber. Biochemical studies were conducted spectrophotometrically for the tissues of the uvea (the iris and the ciliary body) and the aqueous humour. The activity of antioxidant enzymes (glutathione peroxidase, superoxide dismutase, and catalase) was determined. Parametric statistical tests with Student's t-test using the Statistica package were used to process the data.
Results. Ocular hypertension in experimental non-infectious anterior uveitis resulted in a more intensive decrease in the activity of the enzymatic antioxidant system (by 19.4 and 20.9% for superoxide dismutase; by 25.5 and 21.9% for glutathione peroxidase; and by 14.3 and 19.6% for catalase) in the aqueous humor and uveal tissues, respectively, compared to the rabbits with uveitis and normal intraocular pressure.
Conclusions. A decrease revealed in the activity of superoxide dismutase, catalase, and, especially, glutathione peroxidase testifies to depletion of the enzymatic antioxidant system in experimental conditions of increased peroxidation in the uveal tissues in ocular hypertension. Our findings confirm the assumption that elevated intraocular pressure can be a factor contributing to severe inflammation in the anterior eye. One of the major cell mechanisms inducing such pathological conditions is oxidative stress.
Keywords: ocular hypertension, uveitis, glutathione peroxidase, superoxide dismutase, catalase, rabbits
1.Ermakova NA. [General knowledge on uveitis pathogenesis]. RMZh Klin. Oftalmologiia. 2003;4(4):141-3. In Russian.
2.Novitskaia ES. [Early diagnosis of postuveal glaucoma and uveal ocular hypertension in patients with chronic uveitis]. RMZh Klinicheskaia Oftalmologiia. 2012;4:139-41. In Russian.
3.Slepova OS, Arapiev MU, Lovpache DN, Balatskaya NV, Kulikova IG. Specifics of local and systemic cytokine profile in healthy people of different ages and patients with early stage of primary open-angle glaucoma. National Journal glaucoma. 2016;15(1):3-12. https://doi.org/10.1097/00004647-199605000-00004.
4.Sallam А, Sheth HG, Habot-Wilner Z, Lightman S. Outcome of raised intraocular pressure in uveitic eyes with and without a corticosteroid-induced hypertensive response. Am. J. Ophthlmol. 2009;148(2):207-13.
5.Syrovaya AO, Leontieva FS, Novikova IV, Ivannikova SV. [Biological role of free radicals in development of pathological states]. Mezhdunar. Med. Zhurn. 2012;3:98-104. In Russian.
6.Ustinova EI. [Uveal (inflammatory and post-inflammatory) glaucoma (pathogenesis, clinical picture, classification, treatment)]. Oftalmol. Vedomosti. 2009;2(2):81-91. In Russian.
7.Beishenova GA, Chesnokova NB. [The role of free radical oxidation in uveitis pathogenesis (a literature review)]. Ros. Oftalmol. Zhurn. 2015;9(2):99-105. In Russian.
8.Ielskii VN, Mikheitseva IN. [Dysregulatory aspects of the glaucomatous process (Review of the literature and own investigations.] Zhurn. NAMN Ukrainy. 2011;17(3):235-44. In Russian.
9.Erichev VP, Tumanov VP, Panushkina LA. [Glaucoma and nuerodegererative diseases.] Glaukoma;2012;1:62-8. In Russian.
10.Zavgorodniaia NG, Pasechnikova NV. [Primary glaucoma: A new look at an old problem]. Zaporizhzhia: Orbita-YUG; 2010p. In Russian
11.Flammer J, Orgul S, Costa V. The impect of ocular blood flow in glaucoma. Prog. Retinal. Eye Res. 2002;21:359-93.
12.Kurysheva NI, Vinetskaia MI, Erichev VP, Demchuk ML, Kuryshev SI. [Contribution of free-radical reactions of chamber humor to the development of primary open-angle glaucoma]. Vestn Oftalmol. 1996 Sep-Oct;112(4):3-5. In Russian.
13.Gazirova IR. [The state of oxidation-reduction system in patients with primary open angle glaucoma]. Kazanskii Med. Zhurn. 2012;3:488-90. In Russian.
14.Rupali V, Tsai JC, Kolko М. The role of inflammation in the pathogenesis of glaucoma. Surv. Ophthalmol. 2013;58(4):190.
15.Likhvantseva VG, Gabibov AA, Solomatina MV, Belogurov AA, Korosteleva EV, Vygodin VA. The role of immune reactions in the pathogenesis of optic neuropathy in normal tension glaucoma. National Journal glaucoma. 2014;13(2):17-28. In Russian.
16.Wang YY. Experimental study of carbomer glaucoma model in rabbits by injecting different location in anterior chamber. Ophthalmol. 2009;45:1-95.
17.Mikheitseva IM, Bondarenko NV, Kolomiichuk SG, Siroshtanenko TI. Oxidation and peroxidation in the uvea of the rabbit eyes with experimental uveitis and ocular hypertension. J.ophthalmol.(Ukraine).2019;2:55-60.
18.Makarenko EV. [Comprehensive determination of superoxide dismutase and glutathione reductase activity in erythrocytes in patients with chronic liver disease]. Lab. Delo. 1988;11:48-50. In Russian.
19.Koroliuk MA, Ivanova LI, Mayorova IG, Tokarev VE. [A method for determining the catalase activity]. Lab. Delo. 1988;1:16-8. In Russian.
20.Model MA. [On determining the activity of glutathione peroxidase]. Vopr. Med. Khimii. 1989;4:132-3. In Russian.
21.Rebrova OYu. [Statistical analysis of medical data. The use of software package STATISTICA]. M.: Media Sfera; 2002. 312 p. In Russian.
The authors certify that they have no conflicts of interest in the subject matter or materials discussed in this manuscript.