Received: 05 April 2018; Published-online: 23 April 2019

Spectroscopic analysis of intraocular fluid in patients with cataract and pseudoexfoliation syndrome-associated glaucoma

P.A. Bezditko,1 Dr Sc (Med), Prof.; V.O. Melnyk,2 Cand Sc (Med); S.V. Kolotilov,3 Dr Sc (Chem)

1  Kharkiv National Medical University; Kharkiv (Ukraine)

2 Visiobud+ Clinic LLC; Kyiv (Ukraine)

3 Pisarghevskiy Institute of Physical Chemistry of NAS of Ukraine; Kyiv (Ukraine)

E-mail: volo_mel@ukr.net, visiobud@ukr.net

Background: Pseudoexfoliation syndrome (PES) is a risk factor for the development of primary open angle glaucoma (POAG). Impaired intraocular fluid (as reflected by the chemical composition and physical properties, velocity and viscosity, of this fluid) is a major factor for elevated intraocular pressure (IOP).

Purpose: To assess anterior eye characteristics and intraocular fluid protein levels in patients with PES and to compare them with those in patients with glaucoma and patients with cataract only.

Materials and Methods: We assessed anterior eye characteristics (anterior chamber depth and crystalline lens size) and protein levels in the intraocular fluid obtained intraoperatively during lens exchange in patients with PES. These data were compared with those of patients with glaucoma and patients with cataract only.

Results: The changes in anterior eye characteristics and intraocular fluid protein levels in patients with cataract plus PES were similar to those in patients with glaucoma, but not to those in patients with cataract without PES.

Conclusion: Study findings advocate for early surgical cataract extraction to avoid the development of glaucoma in patients with PES.

Keywords:  open angle glaucoma, pseudoexfoliation syndrome, anterior chamber depth, crystalline lens size, spectroscopy of the intraocular fluid


1. Armstrong JJ, Wasiuta T, Kiatos E, et al. The effect of phacoemulsification on intraocular      pressure and topical medication use in patients with glaucoma: A systematic review and meta-analysis of 3-year data. J Glaucoma. 2017 Jun;26(6):511-522. 

Crossref   PubMed 

2. Al-Mugheiry TS, Cate H, Clark A, et al. Microinvasive glaucoma stent surgery (MIGS) with concomitant phakoemulsification cataract extraction: Outcomes and the learning curve.  J Glaucoma. 2017 Jul;26(7):646-651. 


3. Poley BJ, Lindstrom RL, Samuelson TW. Long-term effect of phacoemulsification with intraocular lens implantation in normotensive and ocular hypertensive eyes. J Cataract Refract Surg. 2008 May;34(5):735-42.

Crossref   PubMed

4. Zetterström C, Behndig A, Kugelberg M, et al. Changes in intraocular pressure after cataract surgery: analysis of the Swedish National cataract register data. J Cataract Refract Surgery. 2015 Aug;41(8):1725-9.

Crossref    PubMed

5. Issa de Fendi L, Cena de Oliveira T, Bigheti Pereira C, et al. Additive effect of risk factors for trabeculectomy failure in glaucoma patients. J Glaucoma. 2016 Oct;25(10):e879-e883.

Crossref    PubMed

6. Gabelt BT, Kaufman PL. Aqueous humor hydrodynamics. In: Hart WM, editor. Adler’s Physiology of the eye. 9th ed. St Louis: MO: Mosby; 2003.

7. Koliakos GG, Konstas AGP, Holló G. Biochemistry and genetics of exfoliation syndrome. In: Holló G, Konstas AGP, editors. Exfoliation Syndrome and Exfoliative Glaucoma. 3rd ed. Savona: PubliComm; 2012.


8. Kolotilov SV, Melnyk VO, Lytvynenko AS, et al. Comparison of Spectroscopic Properties of Intraocular Fluid in Patients with Cataract and Primary Open-Angle Glaucoma. Fiziol Zh. 2016;62(5):62-8.

Crossref    PubMed 

9. Mel’nik VO,   Kots-Gotlib NV, Vladiuk RL, Hurzhii OO. Assessment of the efficacy of the combined surgical technique for primary open-angle glaucoma and age-related cataract. J Ophthalmol (Ukraine).2016;3:28-30.




The authors certify that they have no conflicts of interest in the subject matter or materials discussed in this manuscript.