J.ophthalmol.(Ukraine).2018;1:60-66.

https://doi.org/10.31288/oftalmolzh201816066

Effect of different modes of high-frequency electric current on structural changes in the optic nerve in rabbits

N.F. Bobrova 1, Dr. Sc. (Med.), Prof.; V.V. Vit  1, Dr. Sc. (Med.), Prof.;  T.A. Sorochinskaya 1, Cand. Sc. (Med.); N.I. Molchanyuk 1, Cand. Sc. (Med.); I.M. Levitsky 2, ophthalmologist

1 Filatov Institute of Eye Diseases and Tissue Therapy of the NAMS of Ukraine;

Odessa (Ukraine)

2 AILAZ Medical Center;

Kiev (Ukraine)

E-mail: filatovbobrova@gmail.com         

An original technique for enucleation of the eyeball with advanced retinoblastoma using high-frequency electric welding of biological tissues (HFEWBT) was developed in SI “Filatov Institute of Eye Diseases and Tissue Therapy of NAMS of Ukraine” in 2013.

Purpose. To study in experiment the effect of different modes of electric high-frequency current on structural changes of the rabbit’s optic nerve and the extension of the structural changes after eye enucleation. 

Material and Methods. 14 Chinchilla rabbits (21 eyes) were enucleated the eyeball using an EK-300M1 unit. We used two modes of the HF-current exposure for transection of the optic nerve: Group 1 (11 eyes), a cutting mode (current intensity, 1.5 A; voltage, 200 V; power, 350 W; frequency, 66 kHz; exposure, up to 3 sec); Group 2 (10 eyes), a welding (current intensity, up to 0.3 A; voltage, 40-60 V; frequency, 66 kHz; exposure, up to 3 sec) + cutting mode. Fragments of the optic nerve excised during the surgery were studied light-optically and ultrastructurally.

Results. Using both cutting and welding+cutting modes of the HFEVBT method for the transection of the optic nerve caused the development of dry necrosis in the parenchyma and sheaths of the optic nerve not only in the site of exposure but also at the distance which depended on the mode used. Herewith, the welding+cutting mode caused necrosis, growing by the Day 7 up to 3-4 mm wide, which two times exceeded the necrosis extension when using the cutting mode. Degeneration and gliosis of the optic nerve in Group 2 (welding+cutting) occurred within the greater area than that in Group 1 (cutting), reaching the optic disc and sensory retina.

Conclusions. We believe that the extension of the destructive changes when using the welding+cutting HFEWBT mode can increase the ablasticity of enucleation of the eyes with retinoblastoma at high risk of optic nerve invasion.

Key-words: optic nerve, electric welding, high frequency current, experiment 

References

  1. Ivanova OI, Zelnichenko AT, Kunkin DD et al. [Experience of using a high- frequency electric welding unit EK-300M1 in surgery]. Avtomaticheskaya svarka. 2012; 11: 58-60 p. Russian.   
  2. Pasyechnikova NV, Bobrova NF, Naumenko VA, Sorochinskya TA, Levitskiy IM. [Improvement of ablastics of the eyeball enucleation in retinoblastoma]. Oftalmol Zh. 2013;6:62-8. Russian. 
  3. Pasechnikova NV, Bobrova NF, Naumenko VA et al. [A technique of the eyeball enucleation in retinoplastoma]. Patent of Ukraine No u 2014 05175 from 10.10.2014.  Bul. No 9. Russian.
  4. Pasechnikova NV, Vit VV, Pukhlik ES, Naumenko VA, Maletskiy AP, Chebotarev EP. [Peculiarities of the changes of the optic nerve and conjunctiva after exposure to high- frequency electric welding of biological tissues in enucleation of the eyeball in experiment]. Oftalmol Zh.2014;3:81-88. Russian. 
  5. Pasechnikova NV, Maletskiy AP, Naumenko VA et al. [A technique of the  eyeball  enucleation using  the  high-frequency electric welding of biological tissueс]. Patent 46981U, MPK (2009) A61F 9/007. /; Patent of Ukraine № u200907986; from11.01.2010, Bul. No 1. Russian.
  6. Pasechnikova NV, Naumenko VA, Dumbrova NE, Molchanuk NI, Maletskiy AP, Chebotaryov YeS, Pukhlik ES. [Ultrastructural changes in the orbit tissues in enucleation of the eyeball by using the high-frequency electric welding of biological tissues]. Oftalmol Zh.2012;6:85-91. Russian.
  7. Pasechnikova NV, Naumenko VA, Maletskiy AP, Vit VV, Chebotarev EP, Pukhlik ES. [Application of the high-frequency electric welding of soft tissues in enucleation of the eyeball for uveal melanoma]. Oftalmol Zh.2012;4:62-65. Russian. 
  8. Paton BYe. [Electrical welding of soft tissues in surgery]. Avtomaticheskaya svarka. 2004; 9: 7-11 p. Russian. 
  9. Paton BYe, Lebedev VK, Lebedev AV, Masalov YuA, Ivanova ON, Zakharash MP, Furmanov YuA. Patent 75342 С2, MPK (2006) A61B 17/04 B23K 13/00. [A technique of welding of soft tissues in animals and humans]. No 2002065056; appl. 19.06.2002; publ. 17.04.2006; Bul. No 4. Russian. 
  10. Pukhlik ES. [Clinical and experimental substantiation of application of high-frequency electric welding of biological tissues for enucleation of the eyeball associated with uveal melanoma]. Author’s thesis for the candidate of medical science degree. Odessa; 2016. 19 p. Russian.