J.ophthalmol.(Ukraine).2015;6:17-22.

https://doi.org/10.31288/oftalmolzh201561722

Change of interferons ? and ? levels in the blood of patients with uveitis as a result of combination therapy with the use of cycloferon, decametoxine electrophoresis and microwave therapy

N.V. Konovalova

Chaibi Abderrahim

State Institution "The Filatov Institute of Eye Diseases and Tissue Therapy of the National Academy of Medical Sciences of Ukraine''

Odessa (Ukraine)

Key words: uveitis, medicamentous therapy, cycloferon, decametoxine electrophoresis, microwave therapy, interferons ? and ?, blood plasma. 

Introduction. Searching for new effective pathogenetic approaches to the uveitis treatment is both clinically and socially relevant, considering the pathology high prevalence and disease-induced disability.

Objective. To determine the peculiarities of interferons ? and ? expression in the blood of patients with uveitis as a result of combination therapy with the use of cycloferon, decametoxine electrophoresis and microwave therapy.

Materials and methods. Patients with endogenous uveitis received a combination therapy with the use of cycloferon, decametoxine electrophoresis and microwave therapy (26 persons). Control groups: cycloferon and decametoxine electrophoresis (20 persons) and cycloferon and microwave therapy (13 persons). The level of interferons ? and ? was measured in the blood plasma and the ophthalmological examination was carried out before and after the treatment. Parametric and nonparametric criteria, as well as correlation analysis, were used in statistical data processing.

Results. A significant increase of interferons ? and ? concentration was found as a result of the treatment of patients with uveitis. The increase degree does not depend on the focus of inflammation location and the nature of the clinical course. At the same time, the most significant increases are pronounced the most in severe uveitis.

There was found a reliable correlation between the level of interferons before and after the treatment, as well as the clinical characteristics of the disease. The increase of interferons ? and ? level was determined in various clinical forms of uveitis. The most pronounced increase of interferons ? and ? level was detected as a result of decametoxine electrophoresis and microwave therapy on the background of cycloferon administration. It amounts 56.7% and 33.4% respectively.

Conclusions. The increase of interferons level was detected in the blood of patients after the treatment. The most significant increase occurred as a result of decametoxine electrophoresis and microwave therapy on the background of cycloferon administration. It may be considered as one of the treatment efficacy criteria.

References

1. Glanz S. [Biomedical statistics]. Glanz S.: translation from English. M.: Practice. 1998:459. Russian.

2. Yershov FI, Narovlyansky MV, Mezentseva MV[Early cytokine responses in viral infections]. Tsytokiny i vospalenie. 2004;13(1):3-7. Russian.

3. Instruction for using the kit of reagents for quantitative determination of human alpha interferon in biological fluids and human culture mediums. ZAO "Vector-Best''. Novosibirsk; 30.05.08:22. Russian.

4. Instruction for using the kit of reagents for quantitative determination of human gamma interferon in biological fluids and human culture mediums. ZAO "Vector-Best''. Novosibirsk; 30.05.08:22. Russian.

5. Katargina LA, Khvatova AV, Krichevskaya N and others. [Clinical variations and immunological features of rheumatoid uveitis in children of different age]. Vestnik. oftalmol. 2001;117(4):30-33. Russian.

6. Kovalchuk LV, Gankovskaya LV, Rubakov EI. [System of cytokines]. Moscow; 2000:324. Russian.

7. Konovalova NV, Chaibi Abderrahim. [Level of interferon ? and ? in the blood of patients with uveitis with different clinical characteristics and course of the disease]. Oftalmol. Zh. 2015;3:24-29. Russian.

8. Neverova EA. [Pathogenetic substantiation of ozone use in endogenous uveitis]. Dissertation of Candidate of Medical Sciences 14.00.16 Patologicheskaya fiziologiya. 2007; Saransk:116. Russian.

9. Ospelnikova TP, Mironova TV, Poloskov VV. Gharib FYu, Ershov FI. [Effect of interferon inducers on the cytokine profile]. Tsytokiny i vospalenie. 2014;13(1):37-40. Russian.

10. Roit A, Brostoff J, Mail D.[Immunology]. Transl. from English. M.: Mir. 2000:581. Russian.

11. Ryabicheva TG, Varaksin NA, Timofeev NV, M. Rukavishnikov MY. [Determination of cytokines by ELISA of JSC "Vector-Best"]. Informatsionnyi biulleten Novosti "Vector Best". 2004; 4(34). http://www.vectorbest. ru/nvb/n34/st34_4.htm. Russian.

12. Simbirtsev A. [Cytokines is a new system of regulation of body defense reactions]. Tsytokiny i vospalenie. 2002;11(1):9-16. Russian.

13. Khaitov RM, Pinegin BV, Yarilin AA. [Guidelines of clinical immunology. Diagnosis of diseases of the immune system: a guide for doctors]. M.: GEOTAR Media. 2009:352. Russian.

14. Abbas AK, Lichtman AH, Pillai S. Cellular and molecular immunology. 6th ed: Philadelphia; 2009:581.

15. Mahon CR, Tice D. Clinical laboratory immunology. New Jersey: Upper Side River. 2006:325.