Oftalmol Zh.2014;4:88-94
https://doi.org/10.31288/oftalmolzh201448894
Experimental study of the influence the two-week tamponade with perfluorine organic compounds on the bioelectric functional activity of the retina of the rabbit eye
Zhmurik D. V., Milienko M. V.
Kiev municipal clinical hospital «Center of microsurgery of the eye», Kiev (Ukraine)
Key words. bioelectric activity of the retina, perfluorine organic compounds, «heavy» silicone oil, experiment
Introduction. Perfluorine organic compounds (PFOC) possess valuable qualities for vitreoretinal surgery, and their application for short-term tamponade could expand indications to operative treatment and improve results. However, the unequivocal opinion concerning the effect of PFOC on the functional condition of the retina is not present. It is important to compare the effect of PFOC and «heavy» silicone.
The purpose of the study is determination of the peculiarities of the influence of short-term tamponade with PFOC (14 days) on the bioelectric functional activity of the retina in experiment, comparison of the effect of PFOC and «heavy» silicone in dynamics by carrying out electroretinography (ERG) in the different periods after the end of tamponade (7, 14, 30 days) .
Material and methods. The study was made on 6 Chinchilla rabbits (12 eyes). All animals were performed posterior closed subtotal vitrectomy with the subsequent tamponade (14 days) with PFOC (the right eye) and «heavy» silicone (the left eye). ERG was made in all animals before operative intervention and after the end of tamponade in 7, 14 and 30 days.
Results. While analyzing maximal ERG in 7 days after the end of tamponade with PFOC there was revealed increased amplitude of the wave «a» by 28 % (54.5+6.0pV), increased latency of the wave «b» up to 35.2+4.0 MC and its increased amplitude up to 228.5+6.2 pV (77 %). While analyzing rhythmic 3PT it is revealed that the amplitude of the cone response along the wave N1-P1 has increased by 41 %, (48.0+3.0 pV) and latency time was prolonged by 27 %, (60.0+0.5 MC). In 14 days after the end of tamponade significant changes were not observed. In 30 days the functional activity of the central part of the retina was characterized by delay and reduction in the response amplitude to flash of the photoreceptor layer. Middle layers of the center and periphery of the retina were characterized by deterioration of conductivity and the phenomenon of the hyper-response to the stimulus. The influence of «heavy» silicone on bioelectric activity of the retina is comparable with the influence of PFOC.
Conclusion. There were determined peculiarities of the influence of PFOC tamponade for 14 days on the functional activity of the retina of the rabbit eye in dynamics during 1 month of the follow-up after its removal. There were revealed increased amplitude by 30.1 % of the bioelectric response (the phenomenon of supernormal ERG) of the middle layers of the peripheral retina and by 44 % — of the middle layers of the central retina. The influence of heavy silicone with tamponade during 14 days is comparable with the influence of PFOC: increase of the amplitude response of the middle layers of the peripheral retina by 43 % and central retina — by 64.7 %. More prolonged period is required to restore the bioelectric activity of the retina after 14 day PFOC tamponade and «heavy» silicone oil.
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