Received: 24.06.2022; Accepted: 03.08.2022; Published on-line: 27.10.2022

Using a liposomal ozone-based solution in comprehensive treatment of bacterial keratitis

V. M. Sakovych, O. V. Aleksieieva 

Dnipro State Medical University; Dnipro (Ukraine)

TO CITE THIS ARTICLE: Sakovych VM, Aleksieieva OV. Using a liposomal ozone-based solution in comprehensive treatment of bacterial keratitis. J.ophthalmol.(Ukraine).2022;5:19-22.   http://doi.org/10.31288/oftalmolzh202251922  

Background: Keratitis is an inflammatory eye disease frequently leading to complications, significantly decreased vision and visual disability. Common infectious agents include, in particular, aggressive Gram-negative microflora and opportunistic bacteria. The use of conventional antibacterial therapy does not guarantee a positive treatment effect. Infections caused by antibiotic-resistant germs frequently result in increased hospitalization rates with an increased length of inpatient stay at hospital and can cause severe corneal opacities. Formation of biofilms is a cause of increased antibiotic resistance.

Purpose: To improve the efficacy of treatment of bacterial keratitis by integrating an adjunctive liposomal ozone-based solution into comprehensive treatment.

Material and Methods: Forty-four patients (44 eyes) with bacterial keratitis, aged from 29 to 75 years, were involved in the study. Patients were divided into an ozone group of 21 patients and a control group of 23 patients. All patients were administered antibacterial therapy, mydriatics, desensibilizing agents, tissue preparations and vitamins. In addition, patients of the ozone group received an eye drop of ozone-based solution four times a day for 10 days.

Results: The method allowed for a significant (р < 0.001) reduction in conjunctival discharge, mixed conjunctival injection, corneal edema and inflammatory response, leeading to a 3.3 ± 0.1 day reduction in the length of inpatient stay at the hospital.

Conclusion: Utilizing a liposomal ozone-based solution as an adjunct to the comprehensive treatment of bacterial keratitis is a novel treatment method making it possible to accelerate corneal epithelization and reduction in inflammation, and to shorten the length of treatment.

Keywords: bacterial keratitis, liposomal ozone-based solution, comprehensive treatment, clinical scores



1.Laboratory findings in tear fluid analysis. Ohashi Y, Dogru M, Tsubota K. Clin Chim Acta. 2006 Jul 15;369(1):17-28.

Crossref  PubMed

2.Miller D. Update on the epidemiology and antibiotic resistance of ocular infections.  Middle East Afri J Ophthalmol. 2017;24(1):30-42. doi: 10.4103/meajo.MEAJO_276_16.

3.Ventola CL. The antibiotic resistance crisis: part 1: causes and threats. PT. 2015;40: 277–283.  

4.Ting DSJ, Ho CS, Cairns J, et al. 12-year analysis of incidence, microbiological profiles and in vitro antimicrobial susceptibility of infectious keratitis: the Nottingham Infectious Keratitis Study. Br J Ophthalmol. 2021. 105 (3): 328–333.

Crossref  PubMed

5.Smith NL, Wilson AL, Gandhi J, et al. Ozone therapy: an overview of pharmacodynamics, current research, and clinical utility. Med Gas Res. 2017; 7(3):212–219.

Crossref  PubMed

6.Spadea L, Tonti E, Spaterna A, Marchegiani A. Use of ozone-based eye drops: a series of cases in veterinary and human spontaneous ocular pathologies. Case Rep Ophthalmol. 2018;9: 287–298.

Crossref  PubMed

7.Cagini C, Mariniello M, Messina M, et al. The role of ozonized oil and a combination of tobramycin/dexamethasone eye drops in the treatment of viral conjunctivitis: a randomized clinical trial. Int Ophthalmol. 2020;40(12):3209–3215.

Crossref  PubMed

8.Celenza G, Iorio R, Cracchiolo S, et al. Antimycotic activity of ozonized oil in liposome eye drops against Candida spp. Transl Vis Sci Technol. 2020;9:4.

Crossref  PubMed

9.Kim HS, Noh SU, Han YW, et al. Therapeutic effects of topical application of ozone on acute cutaneous wound healing. J Korean Med Sci. 2009;24(3):368–374.

Crossref  PubMed

10.Patel PV, Gujjari SK. The Morphometrical and Histopathological Changes which were Observed after Topical Ozone Therapy on an Exophytic Fibrous Gingival Lesion: A Case Report. J Clin Diagnostic Res. 2013 Jun;7(6):1239-43.

Crossref  PubMed



Author Contribution: V.M. Sakovych: Conceptualization, Project administration, Investigation, Data curation, Writing-original draft, Writing-review & editing; O.V. Aleksieieva: Conceptualization, Data curation, Writing-original draft

Disclaimer: The opinions presented in this article are those of the authors and do not necessarily represent those of their institutions.

Funding sources: none

Conflict of Interest: V.M. Sakovych declares that he has no conflict of interest. .V. Aleksieieva declares that she has no conflict of interest.