Assessing in vitro cytotoxicity and pH of extracts of synthetic polymers made of cross-linked polyurethane composite with immobilized albucid
N.A. Galatenko1, D.V. Kuliesh1, L.F. Narazhaiko1, V.P. Grytsenko1, T.Iu. Zakashun1, A.P. Maletskyy2, N.M. Bigun3
1 Institute for Chemistry of High-Molecular Compounds, Academy of Science of Ukraine; Kyiv (Ukraine)
2 SI "Filatov Institute of Eye Diseases and Tissue Therapy of the National Academy of Medical Sciences of Ukraine"; Odesa (Ukraine)
3 Lviv Regional Clinical Hospital; Lviv (Ukraine)
TO CITE THIS ARTICLE: Galatenko NA, Kuliesh DV, Narazhaiko LF, Grytsenko VP, Zakashun TIu, Maletskyy AP, Bigun NM. Assessing in vitro cytotoxicity and pH of extracts of synthetic polymers made of cross-linked polyurethane composite with immobilized albucid. J.ophthalmol.(Ukraine).2020;4:56-61. http://doi.org/10.31288/oftalmolzh202045661
Background: Because the prevalence of ocular trauma in Ukraine remains high, restorative and reconstructive surgeries in the orbit, adnexa, and periorbital area are important; their success, however, depends on the quality of implant materials. Previously, we have developed a polymer material made of cross-linked polyurethane (PU) and containing a biologically active substance, albucid; it seems to be a promising implant material.
Purpose: To assess in vitro cytotoxicity and pH of extracts of synthetic polymers made of cross-linked PU composites containing and not containing albucid.
Material and Methods: Cell culture methodology was applied to assess in vitro cytotoxicity of cross-linked PU composites containing and not containing albucid. Subcutaneous adipose tissue of albino Wistar rats was used as a source of cells to induce growth of fibroblasts and fibroblast-like cells under culture conditions. The pH value of water solutions was determined through the study of extracts of samples of PU composites containing and not containing albucid. The extracts were examined for pH using the general-purpose pH meter EV-74 supplied with glass pH electrodes.
Conclusion: It was demonstrated that fibroblast culture in Carrel flasks with controls as well as in those with extracts of samples of PU composites containing and not containing albucid was in a stable growth phase, indicating no toxic effect of the extracts on cultured cells. Polyurethane composites containing albucid were found to have a neutral pH value, which was within the acceptable range defined by hygiene standards.
Keywords: cross-linked polyurethane, albucid, implant, reconstructive surgery on the eye, cytotoxicity, pH value
1.Krasnovid TA. [Ocular trauma under present conditions. Providing urgent care in Ukraine]. Proceedings of the Conference of Ophthalmologists of Chernihiv, Kyiv, and other regions. Chernihiv; 2013. pp. 40-4. Russian.
2.Tselomudryi AI, Venger GE, Pogorelyi DN, Rizvaniuk AV. [Current system of stage-by-stage treatment for combat-related eye injuries in the area of ATO]. Visnyk morskoi meditsyny. 2016;2(71):196-203. Russian.
3.Grusha IaO, Fedorov AA, Dzemeshkevich VV, Blinova IV. [The clinical-and-morphological specificity of using xenopericardium in plasty of the eyelid and orbit]. Vestn Oftalmol. 2004 Sep-Oct;120(5):19-21. Russian.
4.Karaian AS. [One-stage repair of traumatic defects and deformations of the cheekbone, nose, and orbit complex]. [Dr Sc (Med) Dissertation]. Moscow: Central Research Institute for Dentistry and Maxillofacial Surgery; 2015. Russian.
5.Astakhov YuS, Nikolaenko VP, D’yakov VE. [Use of polytetrafluoroethylene implants in ophthalmic surgery]. St Petersburg: Foliant; 2007. Russian.
6.Maletskyy AP, Dubkova VI, Maievskaia OI, Bigun NM. [Results of the use of polymer composite Ikvoban in reconstructive surgery of the orbit and oculo-orbital region]. Oftalmologiia. Vostochnaia Evropa. 2013;special issue:188-97. Russian.
7.Chao DL, Harbour JW. Hydroxyapatite versus polyethylene orbital implants for patients undergoing enucleation for uveal melanoma. Can J Ophthalmol. 2015 Apr;50(2):151-4.
8.Galatenko NA, Rozhnova RA. [Biologically active polymeric materials for medicine]. Kyiv: Naukova Dumka; 2013. Russian.
9.Rozhnova RA, Shilov VV, Galatenko NA. [Structural and morphological studies of biologically active implants with prolonged healing action]. Kompozitsiini polymerni materialy. 2000;22 (2): 146-50. Russian
10.Galatenko NA, Kulyesh DV, Maletskyy AP, Karpenko OS. Soft-tissue response to synthetic polymer implants made of cross-linked polyurethane and containing a biologically active substance, albucid or dacarbazine, in animals. J. Ophthalmol. (Ukraine). 2018; 6:52–8.
The authors certify that they have no conflicts of interest in the subject matter or materials discussed in this manuscript.