Changes in temperature of the ocular surface in the projection of the ciliary body in the early stages of induced non-infectious uveitis in rabbits
Dorokhova O., Zborovska O., Meng Guanjun
SI “The Filatov Institute of Eye Diseases and Tissue Therapy of the National Academy of Medical Sciences of Ukraine”; Odesa (Ukraine)
TO CITE THIS ARTICLE: Dorokhova O., Zborovska O., Meng Guanjun. Changes in temperature of the ocular surface in the projection of the ciliary body in the early stages of induced non-infectious uveitis in rabbits. J.ophthalmol.(Ukraine).2020;3:47-52. http://doi.org/10.31288/oftalmolzh202034752
Background: It is an important challenge to develop objective methods for assessing intraocular inflammation.
Purpose: To determine temperatures of the ocular surface in the projection of the ciliary body in rabbits with induced non-infectious anterior and intermediate uveitis in the first five days of the disease.
Material and Methods: Temperatures of the ocular surface in the projection of the ciliary body were determined in seventeen Chinchilla rabbits with induced non-infectious anterior and intermediate uveitis.
Results: At day 1, there was significant difference in temperature of the ocular surface in the projection of the ciliary body between challenged eyes and eyes of intact rabbits (р=0.002) and between fellow eyes and eyes of intact rabbits (р=0.005), but not (р=0.12) between the challenged eye and the fellow eye (35.7°С (1.1) vs 35.0°С (0.9)). At day 3, temperature for the challenged eye was 36.0°С (0.7), and for the fellow eye, 34.9°С (0.5), and the difference was not significant (р = 0.09), but there was significant difference between challenged eyes and eyes of intact rabbits. At day 5, a significant difference in temperature of the ocular surface appeared between the challenged eye and the fellow eye (36.0°С (0.7) vs 34.7°С (0.5); р=0.04), and there was still a significant difference between challenged eyes and eyes of intact rabbits (р=0.008). There was no significant difference in body temperature between challenged animals at day 1 and intact animals (39.1°С vs 39.1°С; р=0.75) and between challenged animals at day 3 and intact animals (39.2°С vs 39.1°С; р=0.58).
Conclusion: On day 1 of induced non-infectious anterior and intermediate uveitis in rabbits, the temperature in ciliary body projection onto the ocular surface increased to 35.7°С (р = 0.002) for the challenged eyes and to 35.0°С (р=0.05) for the fellow eyes compared to eyes of intact rabbits, which is likely to be caused by the response of the autonomic nervous system in the presence of initiation of inflammatory process. On day 5, a significant difference appeared in the ocular surface temperature between the challenged eyes and fellow eyes (36.0°С vs 34.7°С; р = 0.04), which is likely to be caused by decreased temperature response of the autonomic nervous system as well as by a gradual decrease in temperature of the fellow eye.
Keywords: uveitis, objectively assess inflammation, ocular surface temperature
1.Acharya NR, Tham VM, Esterberg E, et al. Incidence and prevalence of uveitis: results from the Pacific Ocular Inflammation Study. JAMA Ophthalmol. 2013 Nov;131(11):1405-12.
2.Bodaghi B, Cassoux N, Wechsler B, et al. Chronic severe uveitis: etiology and visual outcome in 927 patients from a single center. Medicine (Baltimore). 2001 Jul;80(4):263-70.
3.Nussenblatt RB. The natural history of uveitis. Int Ophthalmol. 1990. 14:303–8.
4.Suttorp-Schulten MS, Rothova A. The possible impact of uveitis in blindness: a literature survey. Br J Ophthalmol. 1996 Sep; 80(9): 844–8.
5.Barisani-Asenbauer T, Maca SM, Mejdoubi L, Emminger W, Machold K, Auer H. Uveitis- a rare disease often associated with systemic diseases and infections- a systematic review of 2619 patients. Orphanet J Rare Dis. 2012 Aug 29;7:57.
6.Bakbardina LM. [Thermometric diagnostics of inflammatory process in the anterior uvea]. [Abstract of a Thesis for the Degree of Cand Sc (Med)]. Odesa: Filatov Institute of Eye Disease and Tissue Therapy; 1988. Russian.
7.Astakhov IuS, Kuztetsova TI. [Laser flare photometry in clinical practice]. Oftalmologicheskie vedomosti. 2016;9(2):36-44. Russian.
8.Guney E, Tugal-Tutkun I. Symptoms and Signs of Anterior Uveitis. US Ophthalmic Rev. 2013;6(1):33-7.
9.Herbort CP, Guex-Crosier Y, de Ancos E, et al. Use of laser flare photometry to assess and monitor inflammation in uveitis. Ophthalmology. 1997 Jan;104(1):64-71.
10.Ladas JG, Wheeler NC, Morhun PJ, et al. Laser flare-cell photometry: methodology and clinical applications. Surv Ophthalmol. 2005 Jan-Feb;50(1):27-47.
11.Tugal-Tutkun I, Herbort CP. Laser flare photometry: a noninvasive, objective, and quantitative method to measure intraocular inflammation. Int Ophthalmol. 2010;30:453–64.
12.Wakefield D, Herbort CP, Tugal-Tutkun I, Zierhut M. Controversies in ocular inflammation and immunology laser flare photometry. Ocul Immunol Inflamm. 2010 Oct;18(5):334-40.
13.Gonzales CA, Ladas JG, Davis JL, Feuer WJ, Holland GN. Relationships Between Laser Flare Photometry Values and Complications of Uveitis. Arch Ophthalmol. 2001 Dec;119(12):1763-9.
14.Yeo TH, Ilangovan S, Keane PA, et al. Discrepancies in assessing anterior chamber activity among uveitis specialists. Jpn J Ophthalmol. 2016 May;60(3):206-11.
15.Chernookova VA. [Clinical and functional patterns of oculo-ocular reflexes in unilateral mechanical ocular trauma]. [Abstract of a Thesis for the Degree of Cand Sc (Med)]. Moscow: Moscow Helmholtz Research Institute for Eye Diseases; 2007. Russian.
16.Dorokhova OE, Zborovska OV, Meng Guanjun. Temperature of the ocular surface in the projection of the ciliary body in rabbits. J. Ophthalmol. (Ukraine). 2020; 2(493):65–9.
17.Penkov MA, Saitov MA, Panchenko NV. [Certificate of authorship No. 1,601,629 (USSR). Method for inducing experimental toxic-and-allergic uveitis]. Issued 23.10.90, Bull. No. 39.
18.Anatychuck LI, Pasyechnikova NV, Zadorozhnyy OS, et al. Original device and approaches to the study of temperature distribution in various eye segments (experimental study). J. Ophthalmol. (Ukraine). 2015;6:50-3.
19.Aksionovа SV, Pyatayev NA, Malkina MV, et al. [Comparative examination of two methods for modeling autoimmune uveitis]. Mordovia University Bulletin. 2017; 27(3):428-39. Russian.
20.McDougal DH, Gamlin PD. Autonomic control of the eye. Compr Physiol. 2015 Jan;5(1):439-73.
21.Sullivan DA, Stern ME, Tsubota K, Dartt DA, Sullivan RM, Bromberg BB (Eds). Lacrimal Gland, Tear Film, and Dry Eye Syndromes 3: Basic Science and Clinical Relevance Part B. Adv Exp Med Biol. Vol. 506. Springer; US, 2002.
22.Stewart M, Webster JR, Stafford KJ, et al. Technical note: Effects of an epinephrine infusion on eye temperature and heart rate variability in bull calves. J Dairy Sci. 2010 Nov;93(11):5252-7.
23.Coles WH, Pandya RK, Anbar M, et al. Ocular surface temperature (ocular thermography) as a predictor of corneal wound healing. Invest Ophthalmol Vis Sci. 1988;29:313.
24.Fatt I, Forester JF. Errors in eye tissue temperature measurements when using a metallic probe. Exp Eye Res. 1972 Nov;14(3):270-6. doi: 10.1016/0014-4835(72)90013-9.
25.Freeman RD, Fatt I. Environmental influences on ocular temperature. Invest Ophthalmol. 1973 Aug;12(8):596-602.
26.Lorget F, Parenteau A, Carrier M, et al. Characterization of the pH and temperature in the rabbit, pig, and monkey eye: key parameters for the development of long-acting delivery ocular strategies. Mol Pharm. 2016 Sep 6;13(9):2891-6.
27.Rosenbluth RF, Fatt I. Temperature measurements in the eye. Exp Eye Res. 1977 Oct;25(4):325-41.
28.Schwartz B. Environmental temperature and the ocular temperature gradient. Arch Ophthalmol. 1965 Aug;74:237-43.
29.Antonchik SL. [Temperature characteristics of the eye in health and some pathological processes]. [Abstract of a Thesis for the Degree of Cand Sc (Biol)]. Tyumen: State Medical Academy; 2005. Russian.
30.Katargina LA. [Endogenous uveitis in infants: Clinical, functional and immunological features and management of complications]. [Abstract of a Dissertation for the Degree of Dr Sc (Med)]. Moscow: Helmholtz Research Institute of Eye Diseases; 1992.
31.Khvatova AV, Katargina LA, Lokhmanov VP, Zibarov IN. [Use of distant thermography in uveitis in children]. Vestn Oftalmol. Sep-Oct 1991;107(5):46-9.
32.Mapstone R. Corneal thermal patterns in anterior uveitis. Br J Ophthalmol. 1968 Dec;52(12):917-21.
33.Safronenkova IA. [Diagnostic value of orbital skin thermography in unilateral endogenous uveitis]. [Abstract of a Thesis for the Degree of Cand Sc (Med)]. Odesa: Filatov Institute of Eye Disease and Tissue Therapy; 1989. Russian.
34.Zadorozhnyy O.S., Savin NV, Buiko AS. Improving the technique for controlled cryogenic destruction of conjunctival tumors located in the projection of the ciliary body onto the sclera: A preliminary report. J Ophthalmol (Ukraine). 2018; 5:60-65.
35.Gokul KC, Gurung DB, Adhikary PR. Тhermal effects of eyelid in human eye temperature model. J Appl Math Informatics. 2014; 32 (5-6): 649–63.
36.Rushton JO, Tichy A, Nell B. Introduction of the use of thermography and thermometry in the diagnosis of uveitis in horses: a pilot project. Vet Rec Open. 2015 Jun 27;2(1):e000089.
The authors certify that they have no conflicts of interest in the subject matter or materials discussed in this manuscript.