Anti-inflammatory cytokines content in different clinical uveitis course complicated by the optic nerve inflammation

N.V. Panchenko, Doctor of Medical Sciences

M.N. Samofalova

M.V. Friantseva

Kharkov National Medical University

Kharkov (Ukraine)

E-mail: panchenko0802@gmail.com

The purpose of this work was to study the content of anti-inflammatory cytokines interleukin-4 (IL-4) and interleukin-10 (IL-10) in different clinical uveitis course complicated by the optic nerve inflammation.

Material and Methods. 132 patients with uveitis complicated by the optic nerve inflammation in the active stage were examined and treated.  Anti-inflammatory cytokines (IL-4, IL-10) were determined in the blood serum of all the patients by immunoenzyme method.

Results. It was found that the content of interleukin-4 in uveitis complicated by the inflammation of the optic nerve was 2.3 times and interleukin-10 - 2.6 times lower in comparison with control group, respectively (p <0.05). We found that the lower concentration of interleukin-10 in the blood serum correlates with the recurrent uveitis complicated by the inflammation of the optic nerve, as evidenced by the revealed strong inverse correlation (r = -0,7327; p <0,05).

Conclusions. It was found, that in the active phase of uveitis complicated by the inflammation of the optic nerve, there was a significant reduction in the content of anti-inflammatory cytokines in the blood compared with the control group: interleukin-4 and interleukin-10. Lower concentration of IL-10 in the blood serum correlates with recurrent disease course.


Key words: uveitis complicated by the inflammation of the optic nerve, interleukin-4, interleukin-10   


1.        Drozdova EA, Tarasova LN, Teplova SN, Alekhina TV. [Immunological characteristics of uveitis in case of systemic diseases]. Vestnik oftalmologii. 2004;120(4):24-26. Russian.

2.        Katargina LA, Khvatova AV. [Endogenous uveitis in children and adolescents]. Meditsina. 2000:320. Russian.

3.        Lacomba MC, Martin CM, Chamond RR et al. Aqueous and serum interferon gamma, interleukin (IL) 2, IL-4, and IL-10 in patients with uveitis. Arch. Ophthalmol. 2000;118:768-772.
        Crossref    Pubmed

4.        Tian L, Lei B, Shao J. et al. AAV2-mediated combined subretinal delivery of IFN-αand IL-4 reduces the severity of experimental autoimmune uveoretinitis. PLoS. One. 2012;7(6):e37995.doi:10.1371/journal.pone.0037995.
        Crossref    Pubmed

5.        Cordero-Coma M, Calleja S.  Serum cytokine profile in adalimumab-treated refractory uveitis patients: decreased IL-22 correlates with clinical responses. Ocul. Immunol. Inflamm. 2013;21,(3):212-219.
        Crossref   Pubmed

6.        Chen W., Zhao B., Jiang R et al. Cytokine expression profile in aqueous humor and sera of patients with acute anterior uveitis. Curr. Mol. Med. 2015;15:511-518.
        Crossref   Pubmed

7.        Li Q., Sun B., Matteson DM et al. Cytokines and apoptotic molecules in experimental melanin-protein induced uveitis (EMIU) and experimental autoimmune uveoretinitis (EAU). Autoimmunity. 1999;30:171-182.
        Crossref   Pubmed

8.        Takase H., Futagami Y., Yoshida T. et al. Cytokine Profile in Aqueous Humor and Sera of Patients with Infectious or Noninfectious Uveitis. Invest. Ophthalmol. Vis. Sci. 2006:47(4):1557-1561.
        Crossref   Pubmed

9.        Rupesh V., Somasheila M., Virender I.  Current approach in diagnosis and management of anterior uveitis. Indian. J. Ophthalmol. 2010;58(1):11-19.
        Crossref   Pubmed

10.     Foster C. Uveitis: A Guide for Teachers and Parents. The Ocular Immunology and Uveitis Foundation. Cambridge, USA. 2004:5-89.

11.     Kaufmann U., Diedrichs-Möhring M., Wildner G. Dynamics of intraocular IFN-γ, IL-17 and IL-10-producing cell populations during relapsing and monophasic rat experimental autoimmune uveitis. PLoS. One. 2012;7(11):e49008. doi: 10.1371/journal.pone.0049008.

12.     Pleyer U, Mondino B.  Uveitis and immunological disorders (Essentials in Ophthalmology). Pleyer U.New York. 2005:126-127.

13.     De Groot-Mijnes J., Rothova A., Van Loon A. et al. Polymerase chain reaction and Goldmann-Witmer coefficient analysis are complimentary for the diagnosis of infectious uveitis. Am. J. Ophthalmol. 2006;141:313-318.

14.     Gratacos J, Collado A., Filella X. et al. Serum cytokines (IL-6, TNF-alpha, IL-1 beta and IFN-gamma) in ankylosing spondylitis: a close correlation between serum IL-6 and disease activity and severity. Rheumatol BrJ. 1994;33:927-931.

15.     Damico FM, Cunha-Neto E, Goldberg AC. et al. T-cell recognition and cytokine profile induced by melanocyte epitopes in patients with HLA-DRB1*0405-positive and -negative Vogt-Koyanagi-Harada uveitis. Invest. Ophthalmol. Vis. Sci. 2005;46:2465-2471.

16.     Smet M., Taylor S, Bodaghi B. et al. Understanding uveitis: The impact of research on visual outcomes. Prog. Retin. Eye. Res. 2011;30:452-470.

17.     Xu H, Rizzo LV, Silver PB, Caspi RR. Uveitogenicity is associated with a Th1-like lymphokine profile: cytokine-dependent modulation of early and committed effector T cells in experimental autoimmune uveitis. Cell. Immunol. 1997;178:69-78.