The influence of quercetin and lipoate on the enzymes of extinction of free-radical forms of oxygen (superoxide dismutase and catalase) in experimental diabetes
O. A. Moroz
The Zakarpatye Regional Clinical Hospital named after A. Nowak; Uzhgorod (Ukraine)
Introduction. The importance of the work is in the investigation of quercetin and lipoate effect in treatment of experimental diabetes.
Objective. To investigate quercetin and lipoate effect on the enzymes of the antioxidant system (superoxide dismutase and catalase) in experimental diabetes
Material and methods. The investigation was made on 55 white rats. The animals were divided into four groups: the 1st — a control one (14 rats), the 2nd — a group under study (14 rats) — animals with diabetes without using drugs; the 3rd — a group under study (12 rats) — animals with diabetes and using of lipoic acid; the 4th — a group under study (15 rats) — animals with diabetes and using of quercetin. Activity of superoxidedismutase and catalase was determined in homogenates of the retina and blood serum.
Results. The application of lipoic acid and quercetin had a marked effect on the rate of enzymatic elimination of highly reactive forms of oxygen in the retina and organism in diabetes mellitus.
Conclusion. 1. The application of the drugs of lipoic acid and quercetin considerably activates the anzymes of the antioxidant system in the retina of white rats under the conditions of stpreptosotocyn diabetes. Activity of catalase in the application of lipoate increased by 53.8 and 52.5 % in 2 and 6 months correspondingly; and in using quercetin — by 50.8 and 46.2 % in comparison with animals without using the drug. Activity of superoxide dismutase in application of lipoic acid was increased by 33.6 % (in 2 months) and 34.5 % (in 6 months), and quercetin effect increased activity of superoxide dismutase by 27.9 % and 29.8 % accordingly. 2. On the whole the application of the lipoic acid has more expressive effect on the enzymatic system of extinction of free-radical forms of oxygen in the eye retina in development of experimental diabetes.
Key words: retina, diabetes, antioxidant system, experiment
1.Balabolkin MI, Nikishova MS, Volkova AK et al. The use of antioxidants from the group of flavonoids in the treatment of diabetic retinopathy in type 2 diabetes mellitus. Klin. Endokrinol. 2003;49(3):3–6. In Russian.
2.Gogina IF, Andriyu LV, Ogranovich OE. Diabetic angio, retino-, neuropathy: pathogenesis, clinical features, treatment. Lviv: Liga Press; 2000. 186 p.
3.Evgrafov VYu. Diabetic Retinopathy: pathogenesis, diagnostics, treatment: Author’s thesis for Doctor of Med. Science. M.; 1996. 47 p.
4.Efimov A, Skorobonskaia N, Zuieva N. Diabetic neuropathy. Liky Ukrainy. 2005;3:21–5. In Russian.
5.Kravxhuk EA. The role of free radical oxidation in the pathogenesis of eye diseases. Vestn Oftalmol. 2004:5:48–51. In Russian.
6.Leus NF. Metabolic mechanisms of development and prospects of medical treatment of diabetic retinopathy. Oftalmol Zh. 2003;5:78–80. In Russian.
7.Nasledov A. SPSS computer analysis of data in psychology and social sciences. Spb.:Piter; 2005. 416 p.
8.Nedzvetskaia OV. Modern trends in the treatment of diabetic retinopathy. Mezhdunar. Med. Zhurnal. 2000;3:56–8. In Russian.
9.Oleinik TV. Effect of vitamin and coenzyme complexes on the enzymatic antioxidant system of the retina in experimental diabetes. Oftalmopl Zh. 2007;6:58–61. In Russian.
10.Pavlyuchenko KP, Mogilevskii SYu, Chuiko AL. State of the enzymatic antioxidant system in the retina in experimental diabetes and use of vitamin B6. Oftalmol Zh. 2011;3:73–8. In Russian.
11.Pasyechnikova NV, Naumenko AV, Zborovska AV. The state of the blood retinal barrier in diabetic retinopathy according fluorometry. Oftalmol Zh. 2008;5:4–7. In Russian.
12.Pasyechnikova NV, Laser treatment in the pathologe of the eye fundus. Kyiv: Naukova Dumka; 2007. 201 p.
13.Sidorova MV. Diabetic Retinopathy. Pathogenesis, clinics, treatment. K.: SMP «AVERS»; 2006. 156 p.
14.Aiello LP, Cahill MT, Wong JS. Systemic considerations in the management of diabetic retinopathy. Am. J. Ophthalmol. 2001;32:760–76.
15.Barber A J. A new view of diabetic retinopathy: a neurodegenerative disease of the eye. Prog. In Neuro-Psychopharm. & Biol. Psych. 2003;27:283–90.
16.Bergmeyer HU. Methoden der enzymatischen Analyse. Herausgegeben von H. U. Bergmeyer. Berlin;1986. 2220 p.
17.Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature. 2001;414:813–20.
18.Fong DS, Aiello LP, Ferris FL, Klein RK. Diabetic retinopathy. Ophthalmol. 2004;27:2540–53.
19.Halliwell B. The role of oxygen radicals in human disease with particular reference to the vascular system. Haemostasis. 1993;23:118–26.
20.Jandric-Balen M, Bozikov V, Bistrovic D. Antioxidant enzymes activity in patients with peripheral vascular disease, with and without presence of diabetes mellitus. Coll. Antropol. 2003;27:735–43.
21.Kowluru RA, Atasi L, Ho YS. Role of mitochondrial superoxide dismutase in the development of diabetic retinopathy. Invest. Ophthalmol. Vis. Sci. 2006;47:1594–9.
22.Kurtul N, Bakan E, Aksoy H, Baykal O. Leukocyte lipid peroxidation, superoxide dismutase and catalase activities of type 2 diabetic patients with retinopathy. Acta Medica (Hradec Kralove). 2005;48:35–8.
23.Lang GE. Pharmacological treatment of diabetic retinopathy.Ophthalmologica. 2007; 221(2):112–7.
24.Muchova J, Liptakova A, Orzaghova Z. Antioxidant systems in polymorphonuclear leucocytes of Type 2 diabetes mellitus. Diabet. Med. 1999;16:74–8.
25.Speicher MA, Danis RP, Criswell M. Pharmacologic therapy for diabetic retinopathy. Expert Opion Emerg Drugs. 2003;8(1):239–50.
26.Ulusu NN, Sahilli M, Avci A. Pentose phosphate pathway, glutathione-dependent enzymes and antioxidant argans: effects of stobadine and vitamin E. Neurochem. Res. 2003;28(6):815–23.
27.Winkler R, Moser M. Alterations of antioxidant tissue defense enzymes and related metabolic parameters in streptozotocin-diabetic rats-effects of iodine treatment. Wien Klin. Wochenschr. 1992;104:409–13.