https://doi.org/10.31288/oftalmolzh201426470

The study of the efficiency of lamellar heterogeneous corneal stroma equivalent transplantation in experiment

N. Pasechnikova, B. Kogan, T. Gladuch, I. Nasinnyk, S. Kolomiychuk,E. Vansovich

State Institution The Filatov Institute of eye diseases and tissue therapy NAMS of Ukraine, Odessa (Ukraine)

Introduction. In recent years, research groups of scientists around the world are working to solve urgent problems of transplantation, and to solve the problem of obtaining donor material pigs were considered to be quite acceptable source of transplants (heart, kidney, liver, pancreatic sells) for human transplantations.

Purpose. To study the effectiveness of the lamellar heterogeneous corneal stroma equivalent (CSE) transplantation in experiment received by various methods.

Material and methods. Corneas of enucleated porcine eyes were used to obtain cell-free equivalents of the corneal stroma through several varieties of desellularization. Experimental studies were performed on 25 rabbits (50 eyes) Chinchilla breed. During the operation lamellar CSE transplantation was held using one of five varieties of desellularization. After the surgery antimicrobial and anti-inflam¬matory therapy was performed.

Results. With the help of special processing techniques 5 CSE embodiments were obtained from experimental animal's corneas. Comparative analysis of the data concerning rabbit's eyes state after ESC transplantation of several varieties allows to place them in a number of clinical indicators of the degree of improvement in the following order: № 1 and № 5, № 2, № 3 and № 4. It should be noted that in the case of CSE 4 transplantation degree of conjunctival discharge, conjunctival hyperemia, inflammatory infiltration, and corneal opacity (graft) was 0 points, which distinguishes it from other kinds of CSE.

Conclusion. Rabbit's eyes unresponsiveness after optimal ESC variant transplantation and high clinical efficacy of this type of keratoplasty confirms the possibility of its use in the clinic.

Key words: heterogeneous corneal transplant, the equivalent of the stroma, efficiency, experiment

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