Oftalmol Zh.2013;4:81-86


Activity of lactate dehydrogenase and glucose — 6 phosphate dehydrogenase in the blood during the experimental burn process in the eye in application of intravenous infusions of cytoflavin

R. I. Chalanova, S. G. Kolomiychuk, M. Mbarki

SI «The Filatov Institute of Eye Diseases and Tissue Therapy NAMS of Ukraine»

Department of eye postburn pathology

Key words: burns of the eye, carbohydrate- phosphoric exchange, cytoflavin, experiment

Introduction. The issues of energy exchange during burn damage of the eye have been studied insufficiently enough and the problem of its correction remains undeveloped.

Purpose. The purpose of the investigations was to study dynamics of the indices of lactate dehydrogenase and glucose -6-phosphate dehydrogenase in the serum and hemolysate of the blood during the experimental burn process in the application of intravenous infusions of cytoflavin.

Materials and methods. The studies were carried out in both eyes of 40 rabbits with modeled isolated severe alkaline burn. 2.5 physiological solutions were intravenously introduced in the control group from 10 to 14 days, while in the experi-mental group — 0.5 ml of cytoflavin in 2.0 ml of the physiological solution. Taking of the blood and chamber moisture for conducting biochemical studies was made on the3rd,7th,14th. 21st, 30th and45th day.

Results. Increased activity of lactate dehydrogenase in the blood serum was revealed in dynamics of the burn process and reduction in the activity of glucose-6-phosphate dehydrogenase in the hemolysate of the blood, which indicated the ac-tivation of the glycolytic processes and suppression of the pentose-phosphate cycle. 

Conclusion. The application of cytoflavin contributes to the earlier restoration of these indices, which results in more favorable course of the reduction processes in the burned cornea.


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